UniversitasDiponegoro (Diponegoro University)|Institutional Repository Tanamantapak dara memiliki bunga yang berwarna merah muda atau putih dengan bercak merah yang ada disekitar tangkainya. Buah Buah yang terdapat pada tanaman tapak dara merupakan jenis buah bumbung menggantung yang memiliki bulu dan didalamnya berisi biji. Biji pada tanaman tapak dara memiliki warna hitam pekat. Manfaat Tapak Dara Kandunganlainnya adalah catharanthine, leurosine, norharman, lochnerine, tetrahydroalstonine, vindoline, vindolinine, akuammine, vincamine, vinleurosin, dan vinrosidin. Berbagai alkaloid ini beracun. Tanda-tanda keracunan tapak dara adalah demam, loyo, dan muntah- muntah dalam tempo 24 jam. cash. ArticlePDF AvailableAbstractp> Morphological and physiological characteristics of Vinca at di fferent water stress levels Vinca Vinca rosea L. is one of the important medicinal crops as the sources of raw materials for traditional medicines. Its alkaloid is believed can reduce the risk of cancer, blood tension and diabetes diseases. Recently, vinca is only grown as ornamental plant in the garden, therefore, its cultivation methods is limited, especially its water stress tolerance, lite purpose of this experiment was to study the effect of water stess on the morphological and physiological characteristics of vinca. A pot experiment was conducted in the plastics house of the Research Institute for Spice and Medicinal Crops from November 1997 to March 1998. Factorial experiment consisted two factors and three replications was arranged in a randomized block design. Ihe irst factor consisted of two vinca varieties, those wee white flowers WFV and red flowers of vinca RFV. While 4 different levels of water stress I 100% ield capacity FC, 2 80% FC, 3 60% FC, and 4 40% FC were used as the second factor. Parameters used for evaluating the effect of teatment consisted of morphological characteristics such as I plant height, 2 number of primary branches, 3 number of leaves, 4 leaf area 5 dry weight of stem, 6 dry weight of leaf, 7 dry weight of flowers and 8 dry weight of roots, and physiological characteristics observed including I percentages of opened stomata and 2 free proline content of leaf. Results showed that interaction between varieties and water stress did not significantly affect the morphological nor physiological characteristics. Varieties significantly affected the morphological characteristics except plant height, however they did not signiicantly affect physiological characteristics. Water stress significantly affected the morphological except dry weight of roots and free proline contents. Under 80% FC, vinca produced the highest growth and dry weight of plant however, the plant growth and dry weight wee inhibited under 60 and 40% FC. Under 40% FC, percentages of opened stomata deceased signiicantly. Moeover, proline content in the leaves signiicantly increased by water stess treatments. Cucumber mosaic virus CMV symptoms are found in Catharanthus roseus, patchouly Pogostemon cablin, cubeb Piper chaba, Jasminum sambac and Java-tea Orthosiphon aristatus; however, their genetic characterization has not been studied. The study aimed to molecularly characterize the CMV isolates from Catharanthus roseus, patchouly, cubeb, Jasminum sambac and Java-tea. Disease plant samples showing mosaic and yellow mosaic symptom s were collected from Petak Pamer Garden, ISMCRI, Bogor. Molecular characterization was carried out by reverse transcription - polymerase chain reaction RT-PCR assay using a specific primer of CMV coat protein gene and DNA sequenced. Sequence analysis was performed using the BLAST, Bioedit, Genedoc, Mega 5 programs. The RT-PCR technique succeeded in amplifying a DNA band measuring 650 bp, according to the prediction of the primary design. BLAST analyses revealed that all of these CMV isolates belonged to subgroup IB. Nucleotide sequence homology of CMV from C. roseus, patchouly, P. chaba, and J. sambac, were more than Based on the phylogenetic tree, these four isolates were closely related to CMV isolate from Japan AB070622. Homology of the nucleotide sequence of CMV from Java-tea with the other four isolates w as below This isolate clustered with CMV isolate from Indonesia AB042294 and was separated with another four isolates according to the phylogeny tree. In the amino acid sequence alignment, Java-tea isolates had five different amino acids compared to the other four isolates. This result indicates the poss i bility of CMV transmission between patchouly , Java-tea, C. roseus and J. sambac, so it must be anticipated to prevent its spread.

perbedaan vinca dan tapak dara